Ralph L. Obendorf

Professor of Crop Physiology

 

Seed Biology
Department of Crop and Soil Sciences
617 Bradfield
Cornell University
Ithaca, NY 14853-1901

Tel. (607) 227-9313
FAX (607) 255-2644
email: rlo1@cornell.edu


EDUCATION

B.S. 1960 (Purdue University)

M.S. 1962 (University of California at Davis)

Ph.D. 1966 (University of California at Davis)

Honorary doctorate 2002 (University of Warmia and Mazury in Olsztyn, Poland)

Ralph L. Obendorf, professor of crop physiology since 1978, joined the Cornell faculty as assistant professor in 1966 and was associate professor 1971-1978. His Ph.D. was in Plant Physiology with a concentration in Biochemistry. He was a visiting scientist at the Institute for Cancer Research, FoxChase Cancer Center in Philadelphia in 1972-73, and 1983 he was a visiting plant physiologist at the Plant Growth Laboratory, University of California at Davis. He was a visiting scientist in Chemical Physiology of Plants, Institut fuer Pflanzenphysiologie, University of Vienna in 1997, and a visiting scholar at the University of California at San Diego in 2004.

AWARDS

PROFESSIONAL ACTIVITIES

Owner/Editor, SEED-BIOLOGY-L@cornell.edu an electronic mailing list to foster an exchange of seed biology research information.

PROFESSIONAL MEMBERSHIPS

RESEARCH

Our seed biology research emphasizes health-related components in seeds; molecular structure and synthesis of galactosyl cyclitols; enzymes and genes regulating pathways of soluble carbohydrate biosynthesis and degradation in seeds; regulation of seed and embryo growth, maturation, and germination in vitro and in planta; somatic embryogenesis, desiccation tolerance, and seed water relations; pectin methyl esterase purification, characterization; cell wall polysaccharides in seeds; role of sugars, cyclitols and methanol in seed deterioration and germplasm preservation; regulation of seed set in buckwheat; and composition of buckwheat milling fractions. Early work characterized imbibitional chilling in soybean, maize and sorghum, stelar lesions and hydrational damage, respiration, energy metabolism and transcription.

Seed Biology Research Group, 2001

TEACHING

PATENTS (View)

SELECTED PUBLICATIONS

Slawinska, J., Kantartzi, S.K. and Obendorf, R.L. (2009) In vitro organogenesis of Fagopyrum esculentum Moench (Polygonaceae) as a method to study seed set in buckwheat. European Journal of Plant Science and Biotechnology 00, 000-000 (tentatively accepted).

Kosina, S.M., Castillo, A., Schnebly, S.R. and Obendorf, R.L. (2009) Soybean seed coat cup unloading on plants with low-raffinose, low-stachyose seed. Seed Science Research 19, 000-000 (in press). doi:10.1017/S0960258509990043

Bystrom, L.M., Lewis, B.A., Brown, D.L., Rodriguez, E. and Obendorf, R.L. (2009) Phenolics, sugars, antimicrobial and free-radical-scavenging activities of Melicoccus bijugatus Jacq. fruits from the Dominican Republic and Florida. Journal of Plant Foods for Human Nutrition 000, 000-000. (in press). doi:10.1007/s11130-009-0119-y

Obendorf, R.L., Zimmerman, A.D., Zhang, Q., Castillo, A., Kosina, S.M., Bryant, E.G., Sensenig, E.M., Wu, J. and Schnebly, S.R. (2009) Accumulation of soluble carbohydrates during seed development and maturation of low-raffinose, low-stachyose soybean. Crop Science 49, 329-341. doi:10.2135/cropsci2008.06.0370

Obendorf, R.L. and Horbowicz, M. (2008) Preparation of fagopyritols and uses therefor. Canadian Patent Number 2,236,641. Date of Patent: July 15, 2008. 59 pages (plus 15 figures).

Obendorf, R.L., Sensenig, E.M., Wu, J., Ohashi, M., O'Sullivan, T.E., Kosina, S.M. and Schnebly, S.R. (2008) Soluble carbohydrates in mature soybean seed after feeding D-chiro-inositol, myo-inositol, or D-pinitol to stem-leaf-pod explants of low-raffinose, low-stachyose lines. Plant Science 175, 650-655. doi:10.1016/j.plantsci.2008.06.013

Obendorf, R.L., Zimmerman, A.D., Ortiz, P.A., Taylor, A.G. and Schnebly, S.R. (2008) Imbibitional chilling sensitivity and soluble carbohydrate composition of low raffinose, low stachyose soybean seed. Crop Science 48, 2396-2403. doi:10.2135/cropsci2007.12.0706

Bystrom, L.M., Lewis, B.A., Brown, D.L., Rodriguez, E. and Obendorf, R.L. (2008) Characterizsation of phenolics by LC-UV/Vis, LC-MS/MS and sugars by GC in Melicoccus bijugatus Jacq. 'Montgomery" fruits. Food Chemistry 111, 1017-1024. doi:10.1016/j.foodchem.2008.04.058

Rutzke, C.F.J., Taylor, A.G. and Obendorf, R.L. (2008) Influence of aging, oxygen, and moisture on ethanol production from cabbage seeds. Journal of the American Society for Horticultural Science 133, 158-164.

Ma, J.M., Horbowicz, M. and Obendorf, R.L. (2005) Cyclitol galactosides in embryos of buckwheat stem-leaf-seed explants fed D-chiro-inositol, myo-inositol, or D-pinitol. Seed Science Research 15, 329-338.

Obendorf, R.L., McInnis, C.E., Horbowicz, M., Keresztes, I. and Lahuta, L.B. (2005) Molecular structure of lathyritol, a galactosyl bornesitol from Lathyrus odoratus seeds, by NMR. Carbohydrate Research 340, 1441-1446.

Gomes, C.I., Obendorf, R.L. and Horbowicz, M. (2005) myo-Inositol, D-chiro-inositol, and D-pinitol synthesis, transport, and galactoside formation in soybean explants. Crop Science 45, 1312-1319.

Horbowicz, M., and Obendorf, R.L. (2005) Fagopyritol accumulation and germination of buckwheat seeds matured at 15, 22, or 30°C. Crop Science 45, 1264-1270.

Ueda, T., Coseo, M.P., Harrell, T.J. and Obendorf, R.L. (2005) A multifunctional galactinol synthase catalyzes the synthesis of fagopyritol A1 and fagopyritol B1 in buckwheat seed. Plant Science 168, 681-690.

Obendorf, R.L., Odorcic, S., Ueda, T., Coseo, M.P. and Vassallo, E. (2004) Soybean galactinol synthase forms fagopyritol B1 but not galactopinitols: Substrate feeding of isolated embryos and heterologous expression. Seed Science Research 14, 321-333.

Obendorf, R.L. and Ueda, T. (2004) Fagopyritol synthase genes and uses thereof. United States Patent Application Publication US 2004/0038357 A1. Published: February 26, 2004. Pages 1-89.

Obendorf, R.L. and Ueda, T. (2004) Fagopyritol synthase genes and uses thereof. International Patent Publication Number WO 2004/037974 A2. International Publication Date: 6 May 2004. Pages 1-170.

Obendorf, R.L. and Horbowicz, M. (2004) Preparation of fagopyritols and uses therefor. United States Patent Number 6,825,173 B2. Issued: November 30, 2004. Pages 1-48.

Obendorf, R.L., Odorcic, S. and Gomes, C.I. (2004) Enhancing health-related cyclitols in soy products (abstract). Journal of Nutrition 134, 1254S.

Obendorf, R.L. and Odorcic, S. (2003) Recent accomplishments and new opportunitites in seed research. pp 447-452 in Nicolas, G., Bradford, K.J., Come, D., and Pritchard, H.W. (Eds) The Biology of Seeds: Recent Research Advances. Wallingford, UK, CABI Publishing. ISBN 085199 653 1.

Odorcic, S. and Obendorf, R.L. (2003) Galactosyl cyclitol accumulation enhanced by substrate feeding of soybean embryos. pp 51-60 in Nicolas, G., Bradford, K.J., Come, D., and Pritchard, H.W. (Eds) The Biology of Seeds: Recent Research Advances. Wallingford, UK, CABI Publishing. ISBN 085199 653 1.

Black, M., Pritchard, H. and Obendorf, R.L. (2002) Damage and tolerance in retrospect and prospect. pp 367-372 in Black, M. and Pritchard, H.W. (Eds) Desiccation and Plant Survival. Wallingford, UK, CABI Publishing.

Slawinska, J. and Obendorf, R.L. (2001) Buckwheat seed set in planta and during in vitro inflorescence culture: evaluation of temperature and water deficit stress. Seed Science Research 11, 223-233.

Steadman, K.J., Fuller, D.J. and Obendorf, R.L. (2001) Purification and molecular structure of two digalactosyl D-chiro-inositols and two trigalactosyl D-chiro-inositols from buckwheat seeds. Carbohydrate Research 331, 19-25.

Steadman, K.J., Burgoon, M.S., Lewis, B.A., Edwardson, S.E. and Obendorf, R.L. (2001) Buckwheat milling fractions: Description, macronutrient composition, and dietary fiber. Journal of Cereal Science 33, 271-278.

Steadman, K.J., Burgoon, M.S., Lewis, B.A., Edwardson, S.E. and Obendorf, R.L. (2001) Minerals, phytic acid, tannin and rutin in buckwheat milling fractions. Journal of the Science of Food and Agriculture 81, 1094-1100.

Taylor, D.P. and Obendorf, R.L. (2001) Quantitative assessment of some factors limiting seed set in buckwheat. Crop Science 41, 1792-1799.

Obendorf, R.L. and Horbowicz, M. (2000) Preparation of fagopyritols and uses therefor. United States Patent Number 6,162,795. Date of Patent: December 19, 2000. 35 pages.

Obendorf, R.L., Steadman, K.J., Fuller, D.J., Horbowicz, M. and Lewis, B.A. (2000) Molecular structure of fagopyritol A1 (O-alpha-D-galactopyranosyl-(1-3)-D-chiro-inositol) by NMR. Carbohydrate Research 328, 623-627.

Steadman, K.J., Burgoon, M.S., Schuster, R.L., Lewis, B.A., Edwardson, S.E. and Obendorf, R.L. (2000) Fagopyritols, D-chiro-inositol, and other soluble carbohydrates in buckwheat seed milling fractions. Journal of Agricultural and Food Chemistry 48, 2843-2847.

Koch, J.L., Horbowicz, M. and Obendorf, R.L. (1999) Methanol, pectin and pectin esterase changes during soybean seed maturation. Seed Science Research 9, 311-320.

Taylor, A.G., Johnson, C.F., Kataki, P.K. and Obendorf, R.L. (1999) Ethanol production from hydrated seeds: A high resolution index of seed quality. Acta Horticulturae 504, 153-160.

Horbowicz, M., Brenac, P., Obendorf, R.L. (1998) Fagopyritol B1, O-alpha-D-galactopyranosyl-(1-2)-D-chiro-inositol, a galactosyl cyclitol in maturing buckwheat seeds associated with desiccation tolerance. Planta 205, 1-11.

Markovic, O. and Obendorf, R.L. (1998) Soybean seed pectinesterase. Seed Science Research 8, 455-461.

Obendorf, R.L. (1998) Buckwheat fagopyritols. pp III-65-III-71. In Campbell, C. and Pryzybylski, R. (Eds) Advances in Buckwheat Research, Seventh International Symposium on Buckwheat, Winnipeg, Manitoba, Canada (August 12-14, 1998). Winnepeg, Manitoba, Canada, Published by Organizing Committee under the auspices of the International Buckwheat Research Association.

Obendorf, R.L., Dickerman, A.M., Pflum, T.M., Kacalanos, M.A. and Smith, M.E. (1998) Drying rate alters soluble carbohydrates, desiccation tolerance, and seedling growth of soybean zygotic embryos during in vitro maturation. Plant Science 132, 1-12.

Obendorf, R.L., Horbowicz, M., Dickerman, A.M., Brenac, P. and Smith, M.E. (1998) Soluble oligosaccharides and galactosyl cyclitols in maturing soybean seeds in planta and in vitro. Crop Science 38, 78-84.

Steadman, K.J. and Obendorf, R.L. (1998) The use of buckwheat bran milling fraction in bread. pp III-21-III-26. In Campbell, C. and Pryzybylski, R. (Eds) Advances in Buckwheat Research, Seventh International Symposium on Buckwheat, Winnipeg, Manitoba, Canada (August 12-14, 1998). Winnepeg, Manitoba, Canada, Published by Organizing Committee under the auspices of the International Buckwheat Research Association.

Szczecinski, P., Gryff-Keller, A., Horbowicz, M. and Obendorf, R.L. (1998) NMR investigation of the structure of fagopyritol B1 from buckwheat seeds. Bulletin of the Polish Academy of Sciences Chemistry 46, 9-13.

Brenac, P., Horbowicz, M., Downer, S.M., Dickerman, A.M., Smith, M.E. and Obendorf, R.L. (1997) Raffinose accumulation related to desiccation tolerance during maize (Zea mays L.) seed development and maturation. Journal of Plant Physiology 150, 481-488.

Brenac, P., Smith, M.E. and Obendorf, R.L. (1997) Raffinose accumulation in maize embryos in the absence of a fully functional Vp1 gene product. Planta 203, 222-228.

Brenac, P., Smith, M.E. and Obendorf, R.L. (1997) Raffinose series oligosaccharides and desiccation tolerance of developing viviparous maize embryos. pp 95-101 in Ellis, R.H., Black, M., Murdoch A.J. and Hong, T.D. (Eds) Basic and Applied Aspects of Seed Biology: Proceedings of the 5th International Workshop on Seeds, Reading, 1995. Dordrecht, The Netherlands, Kluwer Academic Publishers.

Górecki, R.J. and Obendorf, R.L. (1997) Galactosyl cyclitols and raffinose family oligosaccharides in relation to desiccation tolerance of pea and soybean seedlings. pp 119-128 inEllis, R.H., Black, M., Murdoch, A.J. and Hong, T.D. (Eds) Basic and Applied Aspects of Seed Biology: Proceedings of the 5th International Workshop on Seeds, Reading, 1995. Amsterdam, Kluwer Academic Publishers.

Górecki, R.J., Piotrowicz-Cieslak, A., Lahuta, L.B. and Obendorf, R.L. (1997) Soluble carbohydrates in desiccation tolerance of yellow lupin seeds during maturation and germination. Seed Science Research 7, 107-115.

Górecki, R.J., Piotrowicz-Cieslak, A. and Obendorf, R.L. (1997) Soluble sugars and flatulence-producing oligosaccharides in maturing yellow lupin (Lupinus luteus L.) seeds. Seed Science Research 7, 185-193.

Kataki, P.K., Horbowicz, M., Taylor, A.G. and Obendorf, R.L. (1997) Changes in sucrose, cyclitols and their galactosyl derivatives with seed aging. pp 515-522 in Ellis, R.H., Black, M., Murdoch A.J. and Hong, T.D. (Eds) Basic and Applied Aspects of Seed Biology: Proceedings of the 5th International Workshop on Seeds, Reading, 1995. Amsterdam, Kluwer Academic Publishers.

Obendorf, R.L. (1997) Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance (Review Update). Seed Science Research 7, 63-74.

Górecki, R.J., Brenac, P., Clapham, W.M., Willcott, J.B. and Obendorf, R.L. (1996) Soluble carbohydrates in white lupin (Lupinus albus L. cv. Ultra) seed matured at 13° and 28° C. Crop Science 36, 1277-1282.

Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1995) Desiccation tolerance in developing soybean seeds: The role of stress proteins. Physiologia Plantarum 93, 630-638.

Horbowicz, M., Obendorf, R.L., McKersie, B.D., and Viands, D.R. (1995) Soluble saccharides and cyclitols in alfalfa (Medicago sativa L.) somatic embryos, leaflets, and mature seeds. Plant Science 109, 191-198.

Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: Dependence on flatulence-producing oligosaccharides and cyclitols--review and survey. Seed Science Research 4, 385-405.

Obendorf, R.L., Horbowicz, M., and Taylor, D.P. (1993) Structure and chemical composition of developing buckwheat seed. pp. 244-251 in Janick, J. and Simon, J.E. (Ed.) New Crops. John Wiley & Sons, New York. View paper

Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1992) Maturation proteins and sugars in desiccation tolerance of developing soybean seeds. Plant Physiology 100, 225-230.

Horbowicz, M. and R.L. Obendorf. (1992) Changes in sterols and fatty acids of buckwheat endosperm and embryo during seed development. Journal of Agricultural and Food Chemistry 40, 745-750.

Obendorf, R.L. (1992) Relationship of methanol accumulation to preharvest seed deterioration and germplasm preservation. Search 27, 1-12.

Blackman, S.A., Wettlaufer, S.H., Obendorf, R.L. and Leopold, A.C. (1991). Maturation proteins associated with desiccation tolerance in soybean. Plant Physiology 97, 868-874.

Polanowski, A.J. and Obendorf, R.L. (1991) Soybean isocitrate lyase: purification, properties, immunoassay and N-terminal sequence. Plant Physiology and Biochemistry 29, 119-129.

Slawinska, J. and Obendorf, R.L. (1991) Soybean somatic embryo maturation: Composition, respiration, and water relations. Seed Science Research 1, 251-262.

Wettlaufer, S.H. and Obendorf, R.L. (1991) Ureides and amides as nitrogen sources for soybean seed growth and maturation in vitro. Crop Science 31, 1319-1323.

Obendorf, R.L., Koch, J.L., Górecki, R.J., Amable, R.A. and Aveni, M.T. (1990) Methanol accumulation in maturing seeds. Journal of Experimental Botany 411, 489-495.

Saab, I.N., and Obendorf, R.L. (1989) Soybean seed water relations during in situ and in vitro growth and maturation. Plant Physiology 89, 610-616.